By W. Belfield, M. Dearden and G. L. Watt (Auth.)
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Extra resources for A Practical Course in Biology
Heat slowly in a fume cupboard until no more fumes of nitric acid are given out. There should now be a yellowish substance left in the bottom of the dish. Using a glass rod, add 1 drop of very dilute ammonia. A purple colour (murexide) will be produced. Now add a little caustic soda. The purple colour changes to blue. SKIN Exercise investigating the surface anatomy of the skin Method 1. Lay the hand of your partner, palm upwards, on the stage of a binocular microscope, focus a strong light onto it and examine.
Using a small paintbrush paint on the palm of the hand a strip of about 25 mm by 12 mm with Formvar (Fig. 14 (i)). The palm of the hand is one of the few parts of the human body which has no hair. Wait until the Formvar is dry and then stick a piece (0 00 Glass slide (i) Formvar or nail varnish painted onto palm of hand (ii) Sellotape stuck over Formvar or nail varnish (iii) Sellotape peeled off hand bringing Formvar or nail varnish away with it (iv) Formvar or nail varnish stuck down to microscope slide by Sellotape FIG.
As a carbohydrate, a 2% suspension of starch should be used (see p. 163); and as a fat, pure olive oil. Test each substrate before use as described below. Proteins. To 1 ml of the boiled cooled milk add excess of dilute sodium hydroxide solution and a few drops of 1 % copper sulphate solution. A violet colour indicates that proteins are present (biuret test). Starch. To a few drops of the starch suspension add a little iodine solution and note the indigo colour. Olive oil. There is no simple and safe test for this, other than smearing a few drops onto paper and noting the translucent greasy mark.
A Practical Course in Biology by W. Belfield, M. Dearden and G. L. Watt (Auth.)