Advances in protein chemistry Volume 2 by M. L.; Edsall, John T. (Eds) Anson PDF

By M. L.; Edsall, John T. (Eds) Anson

ISBN-10: 0120342022

ISBN-13: 9780120342020

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The order in which the different operations are carried out is often vital to the success of the analysis, and methods of working may have to be modified in accordance with variations in the amino acid composition of the protein being analyzed. Little attention has so far been paid to the effect of partial racemization (cf. para. 3) of amino acids on analytical results obtained by these methods, except in the case of glutamic acid (314). It is by precipitation methods that some of the most accurate and generally accepted figures for the amino acid content of proteins have been obtained.

Supplementary references to the mercury precipitation of histidine are 3 3 4 396, 381, 397, 398. Mercufy precipitation is also used for methionine as abreparative procedure (399; cf. 3994 and for tryptophan both prepam tively and in connection with colorimetric work (cf. para. 4). , 340, 341, 353, 400, 363). ANALYTICAL CHEMISTRY OF THE PROTEINS 45 The Work of Bergmann and Colleagues By the use ,of variously substituted aromatic sulfonic acids, etc. Bergmann and hi colleagues (355, 401407) have introduced a new and powerful method of amino acid analysis.

Among results so far obtained by the use of periodate are estimations of hydroxyamino acids in: Eggdbumin (411,476); Serum proteins (480);Muscle proteins (411); Hemoglobins (476);Casein (473, 474, 475, 476, 411, 479); Collagen, gelatin (473,475,476,481);Elastin (480);Lactalbumin (475,479);Lactoglobulin (474,479, 480); Wool, hair (411,474, 481); Silk proteins (411, 477, 482, 483); Insulin (484);Salmine (476);Various purified enzymes (480);Various plant proteins (411, 474, 485, 481); Myosin and fibrin 173a); Various animal organs (485a); Blood piasma proteins (1744.

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Advances in protein chemistry Volume 2 by M. L.; Edsall, John T. (Eds) Anson

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