By WILLIAM E PAUL
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Additional info for Annual Review of Immunology Volume 3 1985
In a series of careful studies they demonstrated that this DNAbreakdowndid not occur when the same target cells were lysed by complementor by hypotonic lysis, but only whendifferent types of CTL were used (157). DNAbreakdownbegan shortly after the calcium-requiring lethal hit phase began (158). Only about 10 minutes were required to digest it to a size that could escape from the detergent-treated nucleus, although target cell lysis as measuredby chromiumrelease required a considerably longer period (159).
As is the case for lymphotoxins,the chief difficulty in accommodating NKCF into a modelof NK-mediated cytolytic action is its slow kinetics. NKCF cytolytic activity can be detected after 16-20 hours of incubation with target cells, but target cell lysis increasesuntil 40-70hours(179,182,189). has been arguedthat purer and moreconcentratedforms of NKCF maykill morerapidly or that a focusing mechanism operates to increase its local concentration (188). Anotherproblemfor the NKCF modelof NKkilling the unclear relationship betweenNK-target binding and the postulated NKCF secretion, whichcan be triggered by manystimuli (186).
W. 1976. Developments in Delayed-Type Hypersensitivities, 19501975. J. Invest. Dermat. 67:136 Polak, L. 1977. Immunological aspects of contact sensitivity. In Advances in Modern Toxicolo~ly, Vol. 4 : Dermatotoxicology and Pharmacology, ed. F. N. Marzulli, H. I. Maibach,p. 225. Washington DC: Hemisphere Annu. Rev. Immunol. 3:1-30. org by HINARI on 08/27/07. For personal use only. Annu. Rev. Immunol. 3:1-30. org by HINARI on 08/27/07. For personal use only. Rev. 3: 31-58 Annu. Rev. Immunol. 3:31-58.
Annual Review of Immunology Volume 3 1985 by WILLIAM E PAUL